Selelekela sa Diminerale tsa Peptide Trace tse Nyenyane
Karolo ea 1 Histori ea Lisebelisoa tsa Liminerale tse Latelang
E ka aroloa ka meloko e mene ho latela ntshetsopele ya di-extension tsa diminerale tse latelang:
Moloko oa pele: Matsoai a sa tloaelehang a liminerale tse sa tloaelehang, joalo ka sulfate ea koporo, sulfate ea ferrous, zinc oxide, jj; Moloko oa bobeli: Matsoai a asiti ea tlhaho a liminerale tse sa tloaelehang, joalo ka lactate ea ferrous, fumarate ea ferrous, citrate ea koporo, jj.; Moloko oa boraro: Sehlopha sa phepo sa amino acid chelate sa liminerale tse sa tloaelehang, joalo ka zinc methionine, glycine ea tšepe le zinc glycine; Moloko oa bone: Matsoai a protheine le matsoai a manyane a chelating a peptide a liminerale tse sa tloaelehang, joalo ka protheine koporo, protheine tšepe, protheine zinc, protheine manganese, koporo e nyane ea peptide, tšepe e nyane ea peptide, peptide zinc e nyane, peptide manganese e nyane, jj.
Moloko oa pele ke liminerale tse sa tloaelehang tse sa tloaelehang, 'me moloko oa bobeli ho isa ho oa bone ke liminerale tse sa tloaelehang tse entsoeng ka tlhaho.
Karolo ea 2 Hobaneng U Lokela ho Khetha Li-Chelate tse Nyenyane tsa Peptide
Li-chelate tse nyane tsa peptide li na le katleho e latelang:
1. Ha dipeptide tse nyane di kopana le di-ion tsa tshepe, di na le dibopeho tse ngata mme di thata ho di kgorisa;
2. Ha e qothisane lehlokoa le dikanale tsa amino acid, e na le dibaka tse ngata tsa ho monya le lebelo le potlakileng la ho monya;
3. Tšebeliso e fokolang ea matla; 4. Li-depositi tse ngata, sekhahla se phahameng sa tšebeliso le ts'ebetso e ntlafetseng haholo ea tlhahiso ea liphoofolo;
5. E thibelang likokoana-hloko le e thibelang likokoana-hloko;
6. Taolo ea 'mele ea ho itšireletsa mafung.
Palo e kholo ea lithuto e bontšitse hore litšobotsi kapa litlamorao tse boletsoeng ka holimo tsa li-chelate tse nyane tsa peptide li etsa hore li be le menyetla e mengata ea ts'ebeliso le bokhoni ba nts'etsopele, kahoo k'hamphani ea rona qetellong e nkile qeto ea ho nka li-chelate tse nyane tsa peptide e le sepheo sa lipatlisiso le nts'etsopele ea lihlahisoa tsa minerale ea tlhaho ea k'hamphani.
Karolo ea 3 Katleho ea li-chelate tse nyane tsa peptide
1. Kamano pakeng tsa li-peptide, li-amino acid le liprotheine
Boima ba limolek'hule tsa protheine bo feta 10000;
Boima ba limolek'hule tsa peptide ke 150 ~ 10000;
Li-peptide tse nyane, tse boetseng li bitsoa li-peptide tse nyane tsa limolek'hule, li na le li-amino acid tse 2 ~ 4;
Boima bo tloaelehileng ba limolek'hule tsa li-amino acid ke hoo e ka bang 150.
2. Ho hokahanya lihlopha tsa li-amino acid le li-peptide tse chelated le litšepe
(1) Lihlopha tse hokahanyang li-amino acid
Lihlopha tse hokahanyang li-amino acid:
Lihlopha tsa amino le carboxyl holim'a a-carbon;
Lihlopha tsa ketane e ka thoko tsa li-amino acid tse ling, joalo ka sehlopha sa sulfhydryl sa cysteine, sehlopha sa phenolic sa tyrosine le sehlopha sa imidazole sa histidine.
(2) Ho hokahanya lihlopha ka li-peptide tse nyane
Li-peptide tse nyane li na le lihlopha tse hokahanyang tse ngata ho feta li-amino acid. Ha li chelate ka li-ion tsa tšepe, ho bonolo ho li chelate, 'me li ka theha chelation ea meno a mangata, e leng se etsang hore chelate e tsitse haholoanyane.
3. Katleho ea sehlahisoa se senyenyane sa chelate ea peptide
Motheo oa khopolo-taba oa peptide e nyane e khothalletsang ho monngoa ha liminerale tse sa reng letho
Litšobotsi tsa ho monya ha li-peptide tse nyane ke motheo oa khopolo-taba oa ho khothaletsa ho monya ha likarolo tse latelang. Ho ea ka khopolo-taba ea metabolism ea protheine ea setso, seo liphoofolo li se hlokang bakeng sa protheine ke seo li se hlokang bakeng sa li-amino acid tse fapaneng. Leha ho le joalo, lilemong tsa morao tjena, lithuto li bontšitse hore karolelano ea tšebeliso ea li-amino acid lijong tse tsoang mehloling e fapaneng e fapane, 'me ha liphoofolo li feptjoa ka lijo tse nang le homozygous kapa lijo tse nang le protheine e tlase e leka-lekaneng, ts'ebetso e ntle ka ho fetisisa ea tlhahiso e ke ke ea fumanoa (Baker, 1977; Pinchasov et al., 1990) [2,3]. Ka hona, litsebi tse ling li hlahisitse pono ea hore liphoofolo li na le bokhoni bo khethehileng ba ho monya bakeng sa protheine e sa senyehang ka boeona kapa li-peptide tse amanang le eona. Agar (1953)[4] o ile a qala ho hlokomela hore tsela ea mala e ka monya le ho tsamaisa diglycidyl ka botlalo. Ho tloha ka nako eo, bafuputsi ba hlahisitse khang e kholisang ea hore li-peptide tse nyane li ka monya ka botlalo, li tiisa hore glycylglycine e sa senyehang e tsamaisoa le ho monya; Palo e kholo ea li-peptide tse nyane e ka monya ka kotloloho ho potoloha ha 'mele ka mokhoa oa li-peptide. Hara et al. (1984)[5] o boetse a supa hore dihlahiswa tsa protheine tse qetellang tsa tshilo ya dijo tse tswelang pele ka tshilo ya dijo boholo ba tsona ke di-peptide tse nyane ho ena le di-amino acid tse lokolohileng (FAA). Di-peptide tse nyane di ka feta ka hara disele tsa mucosal tsa mala ka botlalo mme tsa kena ka hara potoloho ya madi (Le Guowei, 1996)[6].
Tswelopele ya Diphuputso ya Peptide e Nyenyane e Kgothaletsang ho Monyelwa ha Diminerale tse Latelwang, Qiao Wei, jj.
Li-chelate tse nyane tsa peptide lia tsamaisoa le ho monngoa ka mokhoa oa li-peptide tse nyane
Ho ya ka mokhoa wa ho monya le ho tsamaisa le dibopeho tsa dipeptide tse nyane, diminerale tse nyane di kopana le dipeptide tse nyane kaha di-ligand tse kgolo di ka tsamaiswa kaofela, e leng se thusang haholo ntlafatsong ya matla a baeloji a diminerale tse nyane. (Qiao Wei, et al)
Katleho ea Li-Chelate tse Nyenyane tsa Peptide
1. Ha dipeptide tse nyane di kopana le di-ion tsa tshepe, di na le dibopeho tse ngata mme di thata ho di kgorisa;
2. Ha e qothisane lehlokoa le dikanale tsa amino acid, e na le dibaka tse ngata tsa ho monya le lebelo le potlakileng la ho monya;
3. Tšebeliso e fokolang ea matla;
4. Li-depositi tse ngata, sekhahla se phahameng sa tšebeliso le ts'ebetso e ntlafetseng haholo ea tlhahiso ea liphoofolo;
5. E thibelang baktheria le antioxidant; 6. Taolo ea 'mele oa ho itšireletsa mafung.
4. Kutloisiso e eketsehileng ea li-peptide
Ke ofe ho basebelisi ba babeli ba peptide ea fumanang chelete e ngata?
- Peptide e tlamang
- Phosphopeptide
- Li-reagent tse amanang
- Peptide ea antimicrobial
- Peptide ea 'mele ea ho itšireletsa mafung
- Neuropeptide
- Peptide ea lihormone
- Peptide ea antioxidant
- Li-peptide tsa phepo e nepahetseng
- Li-peptide tsa linoko
(1) Tlhophiso ea li-peptide
(2) Litlamorao tsa 'mele tsa li-peptide
- 1. Lokisa ho leka-lekana ha metsi le electrolyte 'meleng;
- 2. Etsa li-antibodies khahlanong le libaktheria le tšoaetso bakeng sa sesole sa 'mele ho ntlafatsa ts'ebetso ea 'mele ea ho itšireletsa mafung;
- 3. Ho thusa ho folisa maqeba; Ho lokisa ka potlako kotsi ea lisele tsa epithelial.
- 4. Ho etsa dienzyme mmeleng ho thusa ho fetola dijo hore e be matla;
- 5. Lokisa lisele, ntlafatsa metabolism ea sele, thibela ho senyeha ha sele, le ho bapala karolo ea ho thibela mofetše;
- 6. Ho kgothaletsa tlhahiso le taolo ya protheine le dienzyme;
- 7. Lenģosa la bohlokoa la lik'hemik'hale ho fetisa tlhahisoleseling lipakeng tsa lisele le litho tsa 'mele;
- 8. Thibelo ea mafu a pelo le methapo ea mali;
- 9. Laola tsamaiso ea endocrine le methapo.
- 10. Ntlafatsa tsamaiso ea tšilo ea lijo le ho phekola mafu a sa foleng a tšilo ea lijo;
- 11. Ntlafatsa lefu la tsoekere, ramatiki, ramatiki le mafu a mang.
- 12. Tšoaetso e loantšang vaerase, e thibelang botsofali, e felisa li-free radical tse ngata 'meleng.
- 13. Ho matlafatsa ts'ebetso ea hematopoietic, ho phekola khaello ea mali, ho thibela ho bokellana ha liplatelete, e leng se ka ntlafatsang bokhoni ba lisele tse khubelu tsa mali bo tsamaisang oksijene.
- 14. Loantša livaerase tsa DNA ka kotloloho 'me u lebise libaktheria tsa vaerase kotsing.
5. Mosebetsi o habeli oa phepo ea li-chelate tse nyane tsa peptide
Chelate e nyane ea peptide e kena ka har'a sele kaofela 'meleng oa phoofolo, 'meebe e roba tlamo ea chelation ka bo eonaka seleng 'me ea bola hore e be li-ion tsa peptide le tsa tšepe, tse sebelisoang kephoofolo e bapala mesebetsi e 'meli ea phepo e nepahetsenghaholo-holokarolo ea ts'ebetso ea peptide.
Mosebetsi oa peptide e nyane
- 1. Khothaletsa tlhahiso ea protheine liseleng tsa mesifa ea liphoofolo, fokotsa apoptosis, 'me u khothaletse kholo ea liphoofolo
- 2. Ntlafatsa sebopeho sa limela tsa mala le ho khothaletsa bophelo bo botle ba mala
- 3. Fana ka masapo a khabone le ho eketsa ts'ebetso ea li-enzyme tsa tšilo ea lijo tse kang amylase ea mala le protease
- 4. E na le litlamorao tsa khatello ea maikutlo tse khahlanong le oxidative
- 5. E na le thepa ea ho thibela ho ruruha
- 6.……
6. Melemo ea li-chelate tse nyane tsa peptide ho feta li-chelate tsa amino acid
| Liminerale tse chelated tsa amino acid | Liminerale tse nyane tsa peptide tse chelated trace | |
| Theko ea thepa e tala | Lisebelisoa tse tala tsa amino acid e le 'ngoe li tura haholo | Lisebelisoa tse tala tsa keratin tsa Chaena li ngata. Moriri, litlhako le manaka tlhokomelong ea liphoofolo le liprotheine le likhechana tsa letlalo indastering ea lik'hemik'hale ke lisebelisoa tsa protheine tsa boleng bo holimo le tse theko e tlase. |
| Phello ea ho monya | Lihlopha tsa amino le carboxyl li ameha ka nako e le 'ngoe ho chelation ea li-amino acid le likarolo tsa tšepe, li etsa sebopeho sa bicyclic endocannabinoid se tšoanang le sa dipeptides, ntle le lihlopha tsa free carboxyl tse teng, tse ka monngoang feela ka sistimi ea oligopeptide. (Su Chunyang et al., 2002) | Ha dipeptide tse nyane di nka karolo ho chelation, sebopeho sa chelation sa lesale le le leng hangata se thehwa ke sehlopha sa amino sa terminal le oksijene ya peptide bond e haufi, mme chelate e boloka sehlopha sa carboxyl se lokolohileng, se ka monyetswang ka sistimi ya dipeptide, ka matla a ho monya a phahameng haholo ho feta sistimi ya oligopeptide. |
| Botsitso | Li-ion tsa tšepe tse nang le masale a le mong kapa ho feta a nang le litho tse hlano kapa a tšeletseng a lihlopha tsa amino, lihlopha tsa carboxyl, lihlopha tsa imidazole, lihlopha tsa phenol, le lihlopha tsa sulfhydryl. | Ntle le lihlopha tse hlano tse teng tsa ho hokahanya li-amino acid, lihlopha tsa carbonyl le imino li-peptide tse nyane le tsona li ka ameha ho hokahaneng, ka hona li etsa hore li-chelate tse nyane tsa peptide li tsitse ho feta li-chelate tsa amino acid. (Yang Pin et al., 2002) |
7. Melemo ea li-chelate tse nyane tsa peptide ho feta glycolic acid le methionine chelate
| Liminerale tse nang le chelated trace tsa glycine | Methionine e nang le liminerale tse chelated trace | Liminerale tse nyane tsa peptide tse chelated trace | |
| Foromo ea ho hokahanya | Lihlopha tsa carboxyl le tsa amino tsa glycine li ka hokahanngoa le li-ion tsa tšepe. | Lihlopha tsa carboxyl le li-amino tsa methionine li ka hokahanngoa le li-ion tsa tšepe. | Ha e kentsoe chelate ka li-ion tsa tšepe, e ruile ka mefuta e kopaneng 'me ha e kolobisoe habonolo. |
| Mosebetsi oa phepo e nepahetseng | Mefuta le mesebetsi ea li-amino acid ke ntho e le 'ngoe. | Mefuta le mesebetsi ea li-amino acid ke ntho e le 'ngoe. | Themefuta e mengata e fapanengea li-amino acid e fana ka phepo e felletseng haholoanyane, ha li-peptide tse nyane li ka sebetsa ka nepo. |
| Phello ea ho monya | Li-chelate tsa Glycine li na lenoLihlopha tsa carboxyl tse lokolohileng lia hlaha 'me li na le phello e liehang ea ho monya. | Li-chelate tsa methionine li na lenoLihlopha tsa carboxyl tse lokolohileng lia hlaha 'me li na le phello e liehang ea ho monya. | Li-chelate tse nyane tsa peptide li thehiloee na leboteng ba lihlopha tsa carboxyl tse lokolohileng 'me li na le phello e potlakileng ea ho monya. |
Karolo ea 4 Lebitso la Khoebo “Li-Chelate tse Nyenyane tsa Peptide-mineral”
Li-Chelate tse nyane tsa Peptide-mineral, joalo ka ha lebitso le bontša, li bonolo ho li chelate.
E bolela ligand tse nyane tsa peptide, tse sa tlaleng habonolo ka lebaka la palo e kholo ea lihlopha tse hokahanyang, Ho bonolo ho theha chelate ea meno a mangata ka likarolo tsa tšepe, e nang le botsitso bo botle.
Karolo ea 5 Selelekela sa Lihlahisoa tsa Letoto la Li-Chelates tse Nyenyane tsa Peptide-mineral
1. Koporo e nyane e nang le peptide e nyenyane e nang le diminerale tse nyane (lebitso la kgwebo: Koporo Amino Acid Chelate Feed Grade)
2. Tšepe e nyane e nang le peptide e nyenyane e nang le chelate ea tšepe (lebitso la khoebo: Ferrous Amino Acid Chelate Feed Grade)
3. Sinki e nyane e nang le peptide trace e chelated mineral (lebitso la kgwebo: Zinc Amino Acid Chelate Feed Grade)
4. Manganese e nyane e nang le peptide trace e chelated mineral (lebitso la kgwebo: Manganese Amino Acid Chelate Feed Grade)
Koporo Amino Acid Chelate Feed Kereiti
Kereiti ea Fepa ea Ferrous Amino Acid Chelate
Kereiti ea Phepo ea Zinki Amino Acid Chelate
Manganese Amino Acid Chelate Feed Kereiti
1. Kereiti ea Phepo ea Amino Acid Chelate ea Koporo
- Lebitso la Sehlahisoa: Koporo Amino Acid Chelate Feed Kereiti
- Ponahalo: Li-granule tse tala tse sootho
- Litekanyetso tsa fisik'hemik'hale
a) Koporo: ≥ 10.0%
b) Kakaretso ea li-amino acid: ≥ 20.0%
c) Sekhahla sa Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Loto: ≤ 5 mg/kg
f) Kadmium: ≤ 5 mg/kg
g) Mongobo: ≤ 5.0%
h) Botenya: Likaroloana tsohle li feta ka har'a letlooeng la 20, ka boholo ba karoloana e kholo ea letlooeng la 60-80
n=0,1,2,... e bontša koporo e chelated bakeng sa dipeptides, tripeptides, le tetrapeptides
Diglycerin
Sebopeho sa li-chelate tse nyane tsa peptide
Litšobotsi tsa Kereiti ea Phepo ea Amino Acid Chelate ea Koporo
- Sehlahisoa sena ke diminerale tse nang le chelate ea tlhaho feela tse chelated ke mekhoa e khethehileng ea chelate e nang le li-peptide tse nyane tsa limolek'hule tse hloekileng tsa enzymatic e le li-substrate tsa chelate le likarolo tse nyane.
- Sehlahisoa sena se tsitsitse ka lik'hemik'hale 'me se ka fokotsa haholo tšenyo ea sona ho livithamine le mafura, jj.
- Tšebeliso ea sehlahisoa sena e thusa ho ntlafatsa boleng ba phepo. Sehlahisoa se monngoa ka litsela tse nyane tsa peptide le amino acid, ho fokotsa tlholisano le bora le likarolo tse ling tse nyenyane, 'me se na le sekhahla se setle ka ho fetisisa sa ho monngoa le ho sebelisoa ha bio.
- Koporo ke karolo e ka sehloohong ea lisele tse khubelu tsa mali, lisele tse hokahaneng, lesapo, tse amehang 'meleng oa li-enzyme tse fapaneng, li ntlafatsa ts'ebetso ea' mele ea ho itšireletsa mafung, li na le phello ea lithibela-mafu, li ka eketsa boima ba 'mele ba letsatsi le letsatsi, li ntlafatsa moputso oa lijo.
Tšebeliso le Katleho ea Kereiti ea Phepo ea Amino Acid Chelate ea Koporo
| Ntho ea kopo | Tekanyo e khothaletsoang (g/t thepa ea boleng bo felletseng) | Dikahare tse ka hara phepo ya boleng bo felletseng (mg/kg) | Bokgoni |
| Jala | 400～700 | 60～105 | 1. Ntlafatsa tshebetso ya ho ikatisa le dilemo tsa tshebediso ya dikolobe tse tshehadi; 2. Eketsa matla a masea le dikolobe tse nyane; 3. Ntlafatsa sesole sa 'mele le ho hanyetsa mafu. |
| Kolobe e nyenyane | 300～600 | 45～90 | 1. Molemo bakeng sa ho ntlafatsa mesebetsi ea hematopoietic le ea 'mele ea ho itšireletsa mafung, ho eketsa ho hanyetsa khatello ea maikutlo le ho hanyetsa mafu; 2. Eketsa sekhahla sa kgolo le ho ntlafatsa katleho ya phepo haholo. |
| Likolobe tse nontšang | 125 | La 18.5 Pherekhong | |
| Nonyana | 125 | La 18.5 Pherekhong | 1. Ntlafatsa ho hanyetsa khatello ea maikutlo le ho fokotsa lefu; 2. Ntlafatsa puseletso ea lijo le ho eketsa sekhahla sa kholo. |
| Liphoofolo tsa metsing | Tlhapi 40～70 | 6～10.5 | 1. Ho kgothaletsa kgolo, ho ntlafatsa puseletso ya dijo; 2. Ho loantša khatello ea maikutlo, ho fokotsa ho kula le lefu. |
| Li-shrimp tse 150～200 | 22.5～30 | ||
| Phoofolo e rusang letsatsi le letsatsi | Pherekhong 0.75 | 1. Thibela ho fetoha ha manonyeletso a tibial, ho se tsamaye hantle ha "mokokotlo o kobehileng", ho thekesela, tshenyo ya mesifa ya pelo; 2. Thibela ho ruruha ha moriri kapa letlalo, ho ba thata, ho lahleheloa ke ho kobeha ho tloaelehileng, ho thibela ho hlaha ha "matheba a bohlooho" ka har'a selikalikoe sa mahlo; 3. Thibela ho theola boima ba 'mele, letšollo, tlhahiso ea lebese Fokotsa. |
2. Kereiti ea Fepa ea Ferrous Amino Acid Chelate
- Lebitso la Sehlahisoa: Ferrous Amino Acid Chelate Feed Kereiti
- Ponahalo: Li-granule tse tala tse sootho
- Litekanyetso tsa fisik'hemik'hale
a) Tšepe: ≥ 10.0%
b) Kakaretso ea li-amino acid: ≥ 19.0%
c) Sekhahla sa Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Loto: ≤ 5 mg/kg
f) Kadmium: ≤ 5 mg/kg
g) Mongobo: ≤ 5.0%
h) Botenya: Likaroloana tsohle li feta ka har'a letlooeng la 20, ka boholo ba karoloana e kholo ea letlooeng la 60-80
n=0,1,2,...e bontša zinki e chelated bakeng sa dipeptides, tripeptides, le tetrapeptides
Litšobotsi tsa Sehlopha sa Fepa sa Ferrous Amino Acid Chelate
- Sehlahisoa sena ke diminerale tse nyenyane tse qhibilihisitsoeng ka mokhoa o ikhethileng oa ho qhibilihisa tse nang le li-peptide tse nyane tsa limolek'hule tse nyane tse qhibilihisang limela e le li-substrate tse qhibilihisang le likarolo tse nyane tse qhibilihisang;
- Sehlahisoa sena se tsitsitse ka lik'hemik'hale 'me se ka fokotsa haholo tšenyo ea sona ho livithamine le mafura, jj. Tšebeliso ea sehlahisoa sena e thusa ho ntlafatsa boleng ba lijo;
- Sehlahisoa se monngoa ka litsela tse nyane tsa peptide le amino acid, ho fokotsa tlholisano le bora le likarolo tse ling tse nyenyane, 'me se na le sekhahla se setle ka ho fetisisa sa ho monngoa le ho sebelisoa ha bio;
- Sehlahisoa sena se ka feta ka har'a mokoallo oa placenta le matsoele, sa etsa hore lesea le sa tsoa emoloa le phele hantle, sa eketsa boima ba tsoalo le boima ba ho khoesa, 'me sa fokotsa sekhahla sa lefu; Tšepe ke karolo ea bohlokoa ea hemoglobin le myoglobin, e ka thibelang ka katleho anemia ea khaello ea tšepe le mathata a eona.
Tšebeliso le Katleho ea Ferrous Amino Acid Chelate Feed Grade
| Ntho ea kopo | Tekanyo e khothalelitsoeng (thepa ea boleng bo felletseng ea g/t) | Dikahare tse ka hara phepo ya boleng bo felletseng (mg/kg) | Bokgoni |
| Jala | 300～800 | 45～120 | 1. Ntlafatsa tshebetso ya ho ikatisa le nako ya tshebediso ya dikolobe tse tshehadi; 2. ntlafatsa boima boo ba tsoalo, boima ba ho kgwesa le ho lekana ha kolobe e nyane bakeng sa tshebetso e ntle ya tlhahiso nakong e tlang; 3. Ntlafatsa polokelo ea tšepe likolobeng tse anyesang le ho eketsa tšepe lebeseng ho thibela khaello ea tšepe likolobeng tse anyesang. |
| Likolobe tse nyane le likolobe tse nontšitsoeng | Likolobe tse nyane 300～600 | 45～90 | 1. Ho ntlafatsa sesole sa mmele sa malinyane a dikolobe, ho matlafatsa ho hanyetsa mafu le ho ntlafatsa sekgahla sa ho phela; 2. Eketsa sekhahla sa kgolo, ntlafatsa phetoho ya dijo, eketsa boima ba ho kgwesa le ho tshwana ha dithole, le ho fokotsa ho ata ha dikolobe tse nang le mafu; 3. Ntlafatsa boemo ba myoglobin le myoglobin, thibela le ho phekola khaello ea tšepe, etsa hore letlalo la kolobe le be lefubelu 'me ho hlakile hore le ntlafatsa' mala oa nama. |
| Likolobe tse nontšitsoeng 200～400 | 30～60 | ||
| Nonyana | 300～400 | 45～60 | 1. Ntlafatsa phetoho ea lijo, eketsa sekhahla sa kholo, ntlafatsa bokhoni ba ho loantša khatello ea maikutlo le ho fokotsa lefu; 2. Ntlafatsa sekhahla sa ho behela mahe, fokotsa sekhahla sa lehe le robehileng le ho tebisa 'mala oa yolk; 3. Ntlafatsa sekhahla sa manyolo le sekhahla sa ho qhotsa mahe a ho ikatisa le sekhahla sa ho pholoha ha likhoho tse nyane. |
| Liphoofolo tsa metsing | 200～300 | 30～45 | 1. Ho kgothaletsa kgolo, ho ntlafatsa phetoho ya dijo; 2. Ntlafatsa matla a ho thibela kgatello ya maikutlo, fokotsa ho kula le ho shwa. |
3. Kereiti ea Phepo ea Zinki Amino Acid Chelate
- Lebitso la Sehlahisoa: Kereiti ea Phepo ea Zinki Amino Acid Chelate
- Ponahalo: makumane a sootho-mosehla
- Litekanyetso tsa fisik'hemik'hale
a) Zinki: ≥ 10.0%
b) Kakaretso ea li-amino acid: ≥ 20.5%
c) Sekhahla sa Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Loto: ≤ 5 mg/kg
f) Kadmium: ≤ 5 mg/kg
g) Mongobo: ≤ 5.0%
h) Botenya: Likaroloana tsohle li feta ka har'a letlooeng la 20, ka boholo ba karoloana e kholo ea letlooeng la 60-80
n=0,1,2,...e bontša zinki e chelated bakeng sa dipeptides, tripeptides, le tetrapeptides
Litšobotsi tsa Kereiti ea Phepo ea Zinc Amino Acid Chelate
Sehlahisoa sena ke minerale e sa tsoakoang e chelated ke mekhoa e ikgethang ya chelating e nang le di-peptide tse nyane tsa dimolek'hule tse enzymatic tse hlwekileng e le di-substrate tsa chelating le dielemente tse sa tsoakoang;
Sehlahisoa sena se tsitsitse ka lik'hemik'hale 'me se ka fokotsa haholo tšenyo ea sona ho livithamine le mafura, jj.
Tšebeliso ea sehlahisoa sena e thusa ho ntlafatsa boleng ba phepo; Sehlahisoa se monngoa ka litsela tse nyane tsa peptide le amino acid, se fokotsa tlholisano le khanyetso le likarolo tse ling tse nyenyane, 'me se na le sekhahla se setle ka ho fetisisa sa ho monngoa le ho sebelisoa ha bio;
Sehlahisoa sena se ka ntlafatsa sesole sa 'mele, sa khothalletsa kholo, sa eketsa phetoho ea lijo le ho ntlafatsa khanya ea boea;
Zinc ke karolo ea bohlokoa ea li-enzyme tse fetang 200, lisele tsa epithelial, ribose le gustatin. E khothalletsa ho ata ha lisele tsa tatso ka potlako ka har'a mucosa ea leleme le ho laola takatso ea lijo; e thibela libaktheria tse kotsi tsa mala; 'me e na le mosebetsi oa lithibela-mafu, tse ka ntlafatsang ts'ebetso ea ho ntša metsi ha sistimi ea tšilo ea lijo le ts'ebetso ea li-enzyme liseleng le liseleng.
Tšebeliso le Katleho ea Kereiti ea Phepo ea Zinc Amino Acid Chelate
| Ntho ea kopo | Tekanyo e khothalelitsoeng (thepa ea boleng bo felletseng ea g/t) | Dikahare tse ka hara phepo ya boleng bo felletseng (mg/kg) | Bokgoni |
| Likhomo tse tšehali tse immeng le tse anyesang | 300～500 | 45～75 | 1. Ntlafatsa tshebetso ya ho ikatisa le nako ya tshebediso ya dikolobe tse tshehadi; 2. Ntlafatsa matla a lesea le sa tsoa emoloa le malinyane, eketsa ho hanyetsa mafu, le ho etsa hore a be le ts'ebetso e ntle ea tlhahiso mohatong oa morao; 3. Ntlafatsa boemo ba mmele ba dikolobe tse tshehadi tse immeng le boima ba tswalo ya dikolobe tse nyane. |
| Kolobe e anyang, e nyenyane le likolobe tse nontšang | 250～400 | 37.5～60 | 1. Ho ntlafatsa sesole sa 'mele sa malinyane a kolobe, ho fokotsa letšollo le lefu; 2. Ho ntlafatsa tatso, ho eketsa ho ja lijo, ho eketsa sekhahla sa kholo le ho ntlafatsa phetoho ea lijo; 3. Etsa hore boea ba kolobe bo benya 'me u ntlafatse boleng ba setopo le boleng ba nama. |
| Nonyana | 300～400 | 45～60 | 1. Ntlafatsa ho benya ha masiba; 2. ntlafatsa sekhahla sa ho behela, sekhahla sa ho nontsha le sekhahla sa ho qhotsa ha mahe a ho ikatisa, le ho matlafatsa bokgoni ba ho daya mmala ba yolk ya lehe; 3. Ntlafatsa bokgoni ba ho thibela kgatello ya maikutlo le ho fokotsa lefu; 4. Ntlafatsa phetoho ea lijo le ho eketsa sekhahla sa kholo. |
| Liphoofolo tsa metsing | La 300 Pherekhong | 45 | 1. Ho kgothaletsa kgolo, ho ntlafatsa phetoho ya dijo; 2. Ntlafatsa matla a ho thibela kgatello ya maikutlo, fokotsa ho kula le ho shwa. |
| Phoofolo e rusang letsatsi le letsatsi | 2.4 | 1. Ntlafatsa tlhahiso ea lebese, thibela mastitis le ho bola ha mafofo, le ho fokotsa bongata ba lisele tsa 'mele lebeseng; 2. Ho kgothaletsa kgolo, ho ntlafatsa phetoho ya dijo le ho ntlafatsa boleng ba nama. |
4. Kereiti ea Phepo ea Manganese Amino Acid Chelate
- Lebitso la Sehlahisoa: Manganese Amino Acid Chelate Feed Kereiti
- Ponahalo: makumane a sootho-mosehla
- Litekanyetso tsa fisik'hemik'hale
a) Mn: ≥ 10.0%
b) Kakaretso ea li-amino acid: ≥ 19.5%
c) Sekhahla sa Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Loto: ≤ 5 mg/kg
f) Kadmium: ≤ 5 mg/kg
g) Mongobo: ≤ 5.0%
h) Botenya: Likaroloana tsohle li feta ka har'a letlooeng la 20, ka boholo ba karoloana e kholo ea letlooeng la 60-80
n=0, 1,2,...e bontša manganese e chelated bakeng sa dipeptides, tripeptides, le tetrapeptides
Litšobotsi tsa Sehlopha sa Phepo sa Manganese Amino Acid Chelate
Sehlahisoa sena ke minerale e sa tsoakoang e chelated ke mekhoa e ikgethang ya chelating e nang le di-peptide tse nyane tsa dimolek'hule tse enzymatic tse hlwekileng e le di-substrate tsa chelating le dielemente tse sa tsoakoang;
Sehlahisoa sena se tsitsitse ka lik'hemik'hale 'me se ka fokotsa haholo tšenyo ea sona ho livithamine le mafura, jj. Tšebeliso ea sehlahisoa sena e thusa ho ntlafatsa boleng ba lijo;
Sehlahisoa se monngoa ka litsela tse nyane tsa peptide le amino acid, ho fokotsa tlholisano le bora le likarolo tse ling tse nyenyane, 'me se na le sekhahla se setle ka ho fetisisa sa ho monngoa le ho sebelisoa ha bio;
Sehlahisoa sena se ka ntlafatsa sekhahla sa kgolo, sa ntlafatsa phetoho ya dijo le boemo ba bophelo bo botle haholo; mme sa ntlafatsa sekhahla sa ho behela, sekhahla sa ho qhotsa le sekhahla se phetseng hantle sa ho ikatisa ha dikgoho ka ho hlaka;
Manganese ea hlokahala bakeng sa kholo ea masapo le tlhokomelo ea lisele tse hokahaneng. E amana haufi-ufi le li-enzyme tse ngata; 'me e nka karolo metabolism ea lik'habohaedreite, mafura le protheine, ho ikatisa le karabelo ea' mele ea ho itšireletsa mafung.
Tšebeliso le Katleho ea Sehlopha sa Phepo sa Manganese Amino Acid Chelate
| Ntho ea kopo | Tekanyo e khothaletsoang (g/t thepa ea boleng bo felletseng) | Dikahare tse ka hara phepo ya boleng bo felletseng (mg/kg) | Bokgoni |
| Kolobe e ikatisang | 200～300 | 30～45 | 1. Ho khothalletsa ntshetsopele e tloaelehileng ea litho tsa botona le botšehali le ho ntlafatsa ho tsamaea ha peō ea botona; 2. Ntlafatsa bokgoni ba ho ikatisa ba dikolobe tse ikatisang le ho fokotsa ditshitiso tsa ho ikatisa. |
| Likolobe tse nyane le likolobe tse nontšitsoeng | 100～250 | 15～37.5 | 1. Ho molemo ho ntlafatsa mesebetsi ea 'mele ea ho itšireletsa mafung, le ho ntlafatsa bokhoni ba ho loantša khatello ea maikutlo le ho hanyetsa mafu; 2. Ho kgothaletsa kgolo le ho ntlafatsa phetoho ya dijo haholo; 3. Ntlafatsa 'mala le boleng ba nama, 'me u ntlafatse peresente ea nama e se nang mafura. |
| Nonyana | 250～350 | 37.5～52.5 | 1. Ntlafatsa bokgoni ba ho thibela kgatello ya maikutlo le ho fokotsa lefu; 2. Ntlafatsa sekhahla sa ho behela, sekhahla sa ho nontsha le sekhahla sa ho qhotsa mahe a ho ikatisa, ntlafatsa boleng ba khetla ea mahe le ho fokotsa sekhahla sa ho robeha ha khetla; 3. Ho khothalletsa kholo ea masapo le ho fokotsa ho ata ha mafu a maoto. |
| Liphoofolo tsa metsing | 100～200 | 15～30 | 1. Ho kgothaletsa kgolo le ho ntlafatsa bokgoni ba yona ba ho lwantsha kgatello ya maikutlo le ho hanela mafu; 2. Ntlafatsa ho tsamaya ha peō ea botona le sekhahla sa ho qhotsa ha mahe a nontšitsoeng. |
| Phoofolo e rusang letsatsi le letsatsi | Likhomo 1.25 | 1. Thibela ho se sebetse hantle ha motsoako oa mafura le tšenyo ea lisele tsa masapo; 2. Ntlafatsa bokgoni ba ho ikatisa, thibela ho ntsha mpa le ho holofala ha diphoofolo tse tshehadi ka mora ho beleha, fokotsa ho shwa ha manamane le dikonyana, le ho eketsa boima ba diphoofolo tse nyane tse sa tswa tswalwa. | |
| Podi 0.25 |
Karolo ea 6 FAB ea Peptide e Nyenyane-Chelate ea Diminerale
| S/N | F: Litšobotsi tse sebetsang | A: Liphapang tsa tlholisano | B: Melemo e tlisoang ke liphapang tsa tlholisano ho basebelisi |
| 1 | Taolo ea khetho ea thepa e tala | Kgetha hydrolysis ya enzymatic ya semela e hlwekileng ya di-peptide tse nyane | Tšireletseho e phahameng ea baeloji, ho qoba ho ja batho |
| 2 | Theknoloji ea tšilo ea lijo e tataisang bakeng sa enzyme ea baeloji ea protheine e habeli | Karolo e phahameng ea li-peptide tse nyane tsa limolek'hule | "Lipheo" tse ling, tse seng bonolo ho li tlatsa, tse nang le ts'ebetso e phahameng ea baeloji le botsitso bo betere |
| 3 | Theknoloji e tsoetseng pele ea ho fafatsa le ho omisa ka khatello | Sehlahisoa sa granular, se nang le boholo bo lekanang ba likaroloana, ho phalla ha metsi ho betere, ha ho bonolo ho monya mongobo | Netefatsa hore ho bonolo ho e sebelisa, ho kopanya ho tšoana haholoanyane phepong e felletseng |
| Metsi a fokolang (≤ 5%), e leng se fokotsang haholo tšusumetso e bakoang ke livithamine le litokisetso tsa li-enzyme | Ntlafatsa botsitso ba lihlahisoa tsa fepa | ||
| 4 | Theknoloji e tsoetseng pele ea taolo ea tlhahiso | Ts'ebetso e koetsoeng ka botlalo, tekanyo e phahameng ea taolo e iketsang | Boleng bo sireletsehileng le bo tsitsitseng |
| 5 | Theknoloji e tsoetseng pele ea taolo ea boleng | Theha le ho ntlafatsa mekhoa ea saense le e tsoetseng pele ea tlhahlobo le mekhoa ea taolo ea ho lemoha lintlha tse amang boleng ba sehlahisoa, joalo ka protheine e qhibilihang ka har'a asiti, kabo ea boima ba limolek'hule, li-amino acid le sekhahla sa chelating | Netefatsa boleng, netefatsa bokgabane le ho ntlafatsa bokgabane |
Karolo ea 7 Papiso ea Mohlolisani
Tekanyetso ea VS e Tloaelehileng
Papiso ea kabo ea peptide le sekhahla sa chelation ea lihlahisoa
| Lihlahisoa tsa Sustar | Palo ea li-peptide tse nyane (180-500) | Lihlahisoa tsa Zinpro | Palo ea li-peptide tse nyane (180-500) |
| AA-Cu | ≥74% | FUMANEHA-CU | 78% |
| AA-Fe | ≥48% | FUMANEHA-FE | 59% |
| AA-Mn | ≥33% | FUMANEHA-Mn | 53% |
| AA-Zn | ≥37% | FUMANEHA-Zn | 56% |
| Lihlahisoa tsa Sustar | Sekhahla sa Chelation | Lihlahisoa tsa Zinpro | Sekhahla sa Chelation |
| AA-Cu | 94.8% | FUMANEHA-CU | 94.8% |
| AA-Fe | 95.3% | FUMANEHA-FE | 93.5% |
| AA-Mn | 94.6% | FUMANEHA-Mn | 94.6% |
| AA-Zn | 97.7% | FUMANEHA-Zn | 90.6% |
Karolelano ea li-peptide tse nyane tsa Sustar e tlase hanyane ho feta ea Zinpro, 'me sekhahla sa chelation sa lihlahisoa tsa Sustar se phahame hanyane ho feta sa lihlahisoa tsa Zinpro.
Papiso ea litaba tsa li-amino acid tse 17 lihlahisoa tse fapaneng
| Lebitso la li-amino acid | Koporo ea Sustar Chelate ea Amino Acid Kereiti ea Fepa | Li-Zinpro E FUMANEHA koporo | Asiti ea Amino ea Ferrous C ea Sustar Fepa ea helite Sehlopha | PHUMANO EA ZINPRO tšepe | Manganese ea Sustar Chelate ea Amino Acid Kereiti ea Fepa | PHUMANO EA ZINPRO manganese | Zinki ea Sustar Asiti ea Amino Kereiti ea Chelate Feed | PHUMANO EA ZINPRO zinki |
| asiti ea aspartic (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| asiti ea glutamic (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | Pherekhong 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Kakaretso ea li-amino acid (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Ka kakaretso, karolo ea li-amino acid lihlahisoa tsa Sustar e phahame ho feta ea lihlahisoa tsa Zinpro.
Karolo ea 8 Litlamorao tsa tšebeliso
Litlamorao tsa mehloli e fapaneng ea liminerale tse sa reng letho ts'ebetsong ea tlhahiso le boleng ba mahe a likhoho tse behelang nakong ea ho behela ea morao
Tshebetso ea Tlhahiso
- Theknoloji ea chelation e reretsoeng
- Theknoloji ea ho beola emulsification
- Theknoloji ea ho fafatsa le ho omisa ka khatello
- Theknoloji ea ho kenya ka sehatsetsing le ho tlosa mongobo
- Theknoloji e tsoetseng pele ea taolo ea tikoloho
Sehlomathiso A: Mekhoa ea ho Fumana Kabo ea Boima ba Limolek'hule ba Li-peptide
Ho amoheloa ha maemo: GB/T 22492-2008
1 Molao-motheo oa Teko:
E ile ea fumanoa ka chromatography ea ho sefa ea gel e sebetsang hantle haholo. Ke hore, ho sebelisoa ho tlatsa ho nang le masoba e le karolo e sa sisinyeheng, ho latela phapang ea boholo ba boima ba limolek'hule ba likarolo tsa sampole bakeng sa karohano, e fumanoeng tlamong ea peptide ea bolelele ba bolelele ba ho monya ha ultraviolet ba 220nm, ho sebelisoa software e inehetseng ea ts'ebetso ea data bakeng sa ho fumana kabo ea boima ba limolek'hule bo amanang ka chromatography ea ho sefa ea gel (ke hore, software ea GPC), li-chromatogram le data ea tsona li ile tsa sebetsoa, tsa baloa ho fumana boholo ba boima ba limolek'hule bo amanang ba peptide ea soya le sebaka sa kabo.
2. Li-reagent
Metsi a teko a lokela ho fihlela tlhaloso ea metsi a bobeli ho GB/T6682, tšebeliso ea li-reagent, ntle le litokisetso tse khethehileng, li hloekile ka tlhahlobo.
2.1 Di-reagent di kenyeletsa acetonitrile (e hlwekileng ka chromatographically), trifluoroacetic acid (e hlwekileng ka chromatographicallyally),
2.2 Lintho tse tloaelehileng tse sebelisoang karolong ea ho lekanya ea kabo ea boima ba limolek'hule: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Seletsa le thepa
3.1 Chromatograph ea Metsi e Sebetsang ka Botlalo (HPLC): sebaka sa mosebetsi sa chromatographic kapa motlakase o nang le sesebelisoa sa ho lemoha UV le software ea ho sebetsana le data ea GPC.
3.2 Yuniti ea ho sefa le ho tlosa khase ea mokhahlelo o tsamaeang.
3.3 Tekanyo ea elektroniki: boleng bo felletseng 0.000 1g.
Mehato e 4 ea ts'ebetso
4.1 Maemo a Chromatographic le liteko tsa ho ikamahanya le tsamaiso (maemo a litšupiso)
4.1.1 Kholomo ea Chromatographic: TSKgelG2000swxl300 mm×7.8 mm (bophara ba ka hare) kapa likholomo tse ling tsa gel tsa mofuta o tšoanang tse nang le ts'ebetso e ts'oanang e loketseng ho fumana liprotheine le li-peptide.
4.1.2 Mokhahlelo o tsamayang: Acetonitrile + metsi + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Bolelele ba leqhubu ba ho lemoha: 220 nm.
4.1.4 Sekhahla sa phallo: 0.5 mL/motsotso.
4.1.5 Nako ea ho lemoha: metsotso e 30.
4.1.6 Bophahamo ba ente ea sampole: 20μL.
4.1.7 Mocheso oa kholomo: mocheso oa kamore.
4.1.8 E le ho etsa hore sistimi ea chromatographic e fihlelle litlhoko tsa ho lemoha, ho ile ha boleloa hore tlas'a maemo a chromatographic a kaholimo, katleho ea kholomo ea gel chromatographic, ke hore, palo ea lipoleiti (N), e ne e se ka tlase ho 10000 e baloang ho latela litlhōrō tsa maemo a tripeptide (Glycine-Glycine-Glycine).
4.2 Tlhahiso ea li-curve tse tloaelehileng tsa boima ba limolek'hule
Litharollo tse fapaneng tse kaholimo tsa peptide e tloaelehileng ea boima ba limolek'hule tse nang le khatello ea boima ba 1 mg / mL li lokisitsoe ka ho bapisa mekhahlelo e tsamaeang, li kopantsoe ka tekanyo e itseng, ebe li sefshoa ka lera la karolo ea tlhaho le boholo ba pore ea 0.2 μm ~ 0.5 μm 'me tsa kenngoa sampole, ebe ho fumanoa li-chromatogram tsa litekanyetso. Li-curve tsa ho lekanya boima ba limolek'hule tse amanang le tsona le li-equation tsa tsona li fumanoe ka ho rala logarithm ea boima ba limolek'hule bo amanang khahlanong le nako ea ho boloka kapa ka ho khutlela morao ka mola.
4.3 Kalafo ea sampole
Lekanya 10mg ea sampole ka nepo ka har'a flask ea volumetric ea 10mL, eketsa karolo e nyane ea ho sisinyeha, ho sisinyeha ha ultrasonic metsotso e 10, e le hore sampole e qhibilihe ka botlalo le ho tsoakoa, e qhibilihisoe ka karolo ea ho sisinyeha ho ea sekaleng, ebe e sefshoa ka lera la karolo ea tlhaho le nang le boholo ba pore ea 0.2μm ~ 0.5μm, 'me sefiltara se hlahlojoe ho latela maemo a chromatographic ho A.4.1.
5. Ho baloa ha kabo ea boima ba limolek'hule tse amanang
Kamora ho sekaseka tharollo ea sampole e lokisitsoeng ho 4.3 tlas'a maemo a chromatographic a 4.1, boima ba limolek'hule bo amanang ba sampole le sebaka sa kabo ea eona li ka fumanoa ka ho nkela data ea chromatographic ea sampole sebaka ka har'a selikalikoe sa calibration 4.2 ka software ea ts'ebetso ea data ea GPC. Kabo ea boima ba limolek'hule bo amanang ba lipeptide tse fapaneng e ka baloa ka mokhoa oa ho normalization oa sebaka se phahameng, ho latela foromo: X=A/A kakaretso×100
Ka foromong: X - Karolo ea boima ba peptide ea boima ba limolek'hule e amanang le peptide eohle sampoleng, %;
A - Sebaka se phahameng sa peptide e nang le boima ba limolek'hule;
Kakaretso ea A - kakaretso ea libaka tse phahameng tsa peptide e 'ngoe le e 'ngoe ea boima ba limolek'hule, e baloang ho fihlela sebakeng se le seng sa desimali.
6 Ho Pheta-pheta
Phapang e felletseng pakeng tsa liqeto tse peli tse ikemetseng tse fumanoeng tlas'a maemo a ho pheta-pheta ha ea lokela ho feta 15% ea karolelano ea lipalo tsa liqeto tse peli.
Sehlomathiso B: Mekhoa ea ho Fumana Li-Amino Acids tse sa Lefelloeng
Ho amoheloa ha maemo: Q/320205 KAVN05-2016
1.2 Li-reagent le thepa
Asiti ea acetic ea glacial: e hloekile ka tlhahlobo
Asiti ea perchloric: 0.0500 mol/L
Sesupo: Sesupo sa kristale violet sa 0.1% (glacial acetic acid)
2. Ho fumana li-amino acid tse lokolohileng
Disampole di omisitswe ho 80°C ka hora e le nngwe.
Beha sampole ka setshelong se omileng hore e phole ka tlhaho ho fihlela mochesong wa kamore kapa e phole ho fihlela mochesong o ka sebediswang.
Bekha hoo e ka bang 0.1 g ea sampole (e nepahetseng ho isa ho 0.001 g) ka har'a botlolo e omileng ea 250 mL e nang le khoele e otlolohileng.
Tsoela pele ka potlako mohatong o latelang ho qoba hore sampole e se ke ea monya mongobo o tikolohong.
Kenya 25 mL ea glacial acetic acid 'me u kopanye hantle metsotso e seng mekae.
Kenya marotholi a 2 a sesupo sa kristale violet
Titrate ka tharollo e tloaelehileng ea titration ea perchloric acid ea 0.0500 mol / L (±0.001) ho fihlela tharollo e fetoha ho tloha ho pherese ho ea qetellong.
Ngola bophahamo ba tharollo e tloaelehileng e sebelisitsoeng.
Etsa teko e se nang letho ka nako e le 'ngoe.
3. Palo le liphetho
Sekhahla sa amino acid e lokolohileng X ho reagent se hlahisoa e le karoloana ea boima (%) 'me se baloa ho latela foromo: X = C × (V1-V0) × 0.1445/M × 100%, ka foromo ea tne:
C - Boima ba tharollo e tloaelehileng ea perchloric acid ka har'a li-moles ka litara (mol/L)
V1 - Bophahamo bo sebediswang bakeng sa ho lekanya disampole ka tharollo e tloaelehileng ya perchloric acid, ka dimililithara (mL).
Vo - Bophahamo bo sebediswang bakeng sa titration e se nang letho ka tharollo e tloaelehileng ya perchloric acid, ka dimililithara (mL);
M - Boima ba sampole, ka digrama (g).
0.1445: Boima bo tloaelehileng ba li-amino acid tse lekanang le 1.00 mL ea tharollo e tloaelehileng ea perchloric acid [c (HClO4) = 1.000 mol / L].
Sehlomathiso C: Mekhoa ea ho Fumana Sekhahla sa Chelation sa Sustar
Ho amoheloa ha maemo: Q/70920556 71-2024
1. Molao-motheo oa qeto (Fe e le mohlala)
Metswako ya tshepe ya amino acid e na le ho qhibiliha ho tlase haholo ka hara ethanol e sa keneng metsi mme di-ion tsa tshepe tse lokolohileng di qhibiliha ka hara ethanol e sa keneng metsi, phapang ya ho qhibiliha pakeng tsa tse pedi ka hara ethanol e sa keneng metsi e sebedisitswe ho fumana sekgahla sa chelation sa metswako ya tshepe ya amino acid.
2. Li-reagent le Litharollo
Ethanol e sa haellang; tse setseng li tšoana le serapa sa 4.5.2 ho GB/T 27983-2011.
3. Mehato ea tlhahlobo
Etsa liteko tse peli ka nako e le 'ngoe. Bekha 0.1g ea sampole e omisitsoeng ho 103±2℃ ka hora e le 'ngoe, e nepahetseng ho 0.0001g, eketsa 100mL ea ethanol e sa naeng metsi ho qhala, sefa, sefa masala a hlatsoitsoeng ka 100mL ea ethanol e sa naeng metsi bonyane makhetlo a mararo, ebe u fetisetsa masala ka har'a botlolo ea 250mL e kobehileng, eketsa 10mL ea tharollo ea sulfuric acid ho latela serapa sa 4.5.3 ho GB/T27983-2011, ebe u etsa mehato e latelang ho latela serapa sa 4.5.3 "Futhumatsa ho qhala ebe u tlohela hore e phole" ho GB/T27983-2011. Etsa teko e se nang letho ka nako e le 'ngoe.
4. Ho fumana kakaretso ea tšepe
4.1 Molao-motheo oa qeto o tšoana le serapa sa 4.4.1 ho GB/T 21996-2008.
4.2. Li-reagent le Litharollo
4.2.1 Asiti e tsoakiloeng: Kenya 150mL ea sulfuric acid le 150mL ea phosphoric acid ho 700mL ea metsi 'me u kopanye hantle.
4.2.2 Tharollo ea sesupo sa sodium diphenylamine sulfonate: 5g/L, e lokisitsoeng ho latela GB/T603.
4.2.3 Tharollo e tloaelehileng ea titration ea Cerium sulfate: mahloriso c [Ce (SO4) 2] = 0.1 mol/L, e lokisitsoeng ho latela GB/T601.
4.3 Mehato ea tlhahlobo
Etsa liteko tse peli ka nako e le 'ngoe. Bekha 0.1g ea sampole, e nepahetseng ho 020001g, e behe ka har'a flask ea 250mL ea conical, eketsa 10mL ea asiti e tsoakiloeng, kamora ho qhibiliha, eketsa 30ml ea metsi le marotholi a 4 a tharollo ea sodium dianiline sulfonate indicator, ebe u etsa mehato e latelang ho latela serapa sa 4.4.2 ho GB/T21996-2008. Etsa teko e se nang letho ka nako e le 'ngoe.
4.4 Boemeli ba liphetho
Kakaretso ea tšepe X1 ea metsoako ea tšepe ea amino acid mabapi le karolo ea boima ba tšepe, boleng bo hlalositsoeng ka %, bo baliloe ho latela foromo (1):
X1=(V-V0)×C×M×10-3×100
Ka foromo: V - bophahamo ba tharollo e tloaelehileng ea cerium sulfate e sebelisoang bakeng sa titration ea tharollo ea teko, mL;
V0 - tharollo e tloaelehileng ea cerium sulfate e sebelisoang bakeng sa titration ea tharollo e se nang letho, mL;
C - Khokahano ea 'nete ea tharollo e tloaelehileng ea cerium sulfate, mol/L
5. Ho baloa ha dikahare tsa tshepe ka hara chelate
Sekhahla sa tšepe X2 ho chelate ho ya ka karolo ya boima ba tshepe, boleng bo hlahisitsweng ka %, bo badilwe ho ya ka foromo: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
Ka foromo: V1 - bophahamo ba tharollo e tloaelehileng ea cerium sulfate e sebelisoang bakeng sa titration ea tharollo ea teko, mL;
V2 - tharollo e tloaelehileng ea cerium sulfate e sebelisoang bakeng sa titration ea tharollo e se nang letho, mL;
C - Khokahano ea 'nete ea tharollo e tloaelehileng ea cerium sulfate, mol/L;
0.05585 - boima ba tšepe ea ferrous e hlahisoang ka ligrama tse lekanang le 1.00 mL ea tharollo e tloaelehileng ea cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1 - Boima ba sampole, g. Nka karolelano ea lipalo ea liphetho tsa qeto e bapileng e le liphetho tsa qeto, 'me phapang e felletseng ea liphetho tsa qeto e bapileng ha e fete 0.3%.
6. Palo ea sekhahla sa chelation
Sekhahla sa Chelation X3, boleng bo hlahisitsoeng ka %, X3 = X2/X1 × 100
Sehlomathiso C: Mekhoa ea ho Fumana Sekhahla sa Chelation sa Zinpro
Ho amoheloa ha maemo: Q/320205 KAVNO7-2016
1. Li-reagent le thepa
a) Asiti ea acetic ea glacial: e hloekile ka tlhahlobo; b) Asiti ea perchloric: 0.0500mol/L; c) Sesupo: Sesupo sa kristale ea violet ea 0.1% (asiti ea acetic ea glacial)
2. Ho fumana li-amino acid tse lokolohileng
2.1 Disampole di omisitswe ho 80°C ka hora e le nngwe.
2.2 Beha sampole ka setshelong se omileng hore e phole ka tlhaho ho fihlela mochesong wa kamore kapa e phole ho fihlela mochesong o ka sebediswang.
2.3 Bekha hoo e ka bang 0.1 g ea sampole (e nepahetseng ho isa ho 0.001 g) ka har'a botlolo e omileng ea 250 mL e omileng.
2.4 Tsoela pele ka potlako mohatong o latelang ho qoba hore sampole e se ke ea monya mongobo o tikolohong.
2.5 Kenya 25mL ea glacial acetic acid 'me u kopanye hantle metsotso e seng mekae.
2.6 Kenya marothodi a 2 a sesupo sa kristale violet.
2.7 Titrate ka tharollo e tloaelehileng ea titration ea perchloric acid ea 0.0500mol/L (±0.001) ho fihlela tharollo e fetoha ho tloha pherese ho ea ho e tala ka metsotso e 15 ntle le ho fetola 'mala e le ntlha ea ho qetela.
2.8 Ngola bophahamo ba tharollo e tloaelehileng e sebelisitsoeng.
2.9 Etsa teko e se nang letho ka nako e le 'ngoe.
3. Palo le liphetho
Sekhahla sa amino acid e lokolohileng X ho reagent se hlahiswa e le karoloana ya boima (%), e balwang ho ya ka foromo (1): X=C×(V1-V0) ×0.1445/M×100%........(1)
Ka mokhoa o latelang: C - mahloriso a tharollo e tloaelehileng ea perchloric acid ka har'a li-moles ka litara (mol/L)
V1 - Bophahamo bo sebediswang bakeng sa ho lekanya disampole ka tharollo e tloaelehileng ya perchloric acid, ka dimililithara (mL).
Vo - Bophahamo bo sebediswang bakeng sa titration e se nang letho ka tharollo e tloaelehileng ya perchloric acid, ka dimililithara (mL);
M - Boima ba sampole, ka digrama (g).
0.1445 - Boima bo tloaelehileng ba li-amino acid tse lekanang le 1.00 mL ea tharollo e tloaelehileng ea perchloric acid [c (HClO4) = 1.000 mol / L].
4. Palo ea sekhahla sa chelation
Sekhahla sa chelation sa sampole se hlahiswa e le karoloana ya boima (%), e balwang ho ya ka foromo (2): sekgahla sa chelation = (kakaretso ya amino acid - dikahare tsa amino acid tse se nang amino)/kakaretso ya dikahare tsa amino acid×100%.
Nako ea poso: Loetse-17-2025
